研究実績

2021 |2020 |2019 |2018 |2017 |2016 |2015 |2014 |2013 | |2012 | 2011 | 2010 | 2009 | 2008 | 2007
2006 | 2005 | 2004 | 2003 | 2002 | 2000 | 1999 | 1998 | 1997 | 1996 | 1994

2021年

【原著論文】

Nishinaka-Arai Y, Niwa A, Matsuo S, Kazuki Y, Yakura Y, Hiroma T, Toki T, Sakuma T, Yamamoto T, Ito E, Oshimura M, Nakahata T and Saito MK
Down syndrome-related transient abnormal myelopoiesis is attributed to a specific erythro-megakaryocytic subpopulation with GATA1 mutation
Haematologica, 106(2):635-640, 2021
Pieplow A, Dastaw M, Sakuma T, Sakamoto N, Yamamoto T, Yajima M, Oulhen N, Wessel GM
CRISPR-Cas9 editing of non-coding genomic loci as a means of controlling gene expression in the sea urchin
Developmental Biology, 472:85-97, 2021
Arima Y, Nakagawa Y, Takeo T, Ishida T, Yamada T, Hino S, Nakao M, Hanada S, Umemoto T, Suda T, Sakuma T, Yamamoto T, Watanabe T, Nagaoka K, Tanaka Y, Kawamura YK, Tonami K, Kurihara H, Sato Y, Yamagata K, Nakamura T, Araki S, Yamamoto E, Izumiya Y, Sakamoto K, Kaikita K, Matsushita K, Nishiyama K, Nakagata N, Tsujita K
Murine neonatal ketogenesis preserves mitochondrial energetics by preventing protein hyperacetylation
Nature Metabolism, 3(2):196-210, 2021

【総説・著書】

佐久間哲史、山本 卓
CRISPR最新ツールボックス
実験医学、羊土社, 2021

2020年

【原著論文】

Li J, Hsu A, Hua Y, Wang G, Cheng L, Ochiai H, Yamamoto T, Pertsinidis A
Single-gene imaging links genome topology, promoter–enhancer communication and transcription control
Nature Structural & Molecular Biology, 7(11):1032-1040, 2020
Sanoh S, Hanada H, Kashiwagi K, Mori T, Goto-Inoue N, Suzuki KT, Mori J, Nakamura N, Yamamoto T, kiKitamura S, Kotake Y, Sugihara K, Ohta S, Kashiwagi A
Amiodarone bioconcentration and suppression of metamorphosis in Xenopus
Aquatic Toxicology, 228:105623, 2020
Kurita T, Moroi K, Iwai M, Okazaki K, Shimizu S, Nomura S, Saito F, Maeda S, Takami A, Sakamoto A, Ohta H, Sakuma T and Yamamoto T
Efficient and multiplexable genome editing using Platinum TALENs in oleaginous microalga, Nannochloropsis oceanica NIES-2145
Genes to Cells, 25(10):695-702, 2020
Sekiguchi M, Sobue A, Kushima I, Wang C, Arioka Y, Kato H, Kodama A, Kubo H, Ito N, Sawahata M, Hada K, Ikeda R, Shinno M, Mizukoshi C, Tsujimura K, Yoshimi A, Ishizuka K, Takasaki Y, Kimura H, Xing J, Yu Y, Yamamoto M, Okada T, Shishido E, Inada T, Nakatochi M, Takano T, Kuroda K, Amano M, Aleksic B, Yamamoto T, Sakuma T, Aida T, Tanaka K, Hashimoto R, Arai M, Ikeda M, Iwata N, Shimamura T, Nagai T, Nabeshima T, Kaibuchi K, Yamada K, Mori D, Ozaki N.
ARHGAP10, which encodes Rho GTPase-activating protein 10, is a novel gene for schizophrenia risk
Transl Psychiatry, 10(1):247, 2020
Ochiai H, Hayashi T, Umeda M, Yoshimura M, Harada A, Shimizu Y, Nakano K, Saitoh N, Liu Z, Yamamoto T, Okamura T, Ohkawa Y, Kimura H and Nikaido I
Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells
Science Advances, in press, 2020
Yaguchi S, Yaguchi J, Suzuki H, Kinjo S, Kiyomoto M, Ikeo K and Yamamoto T
Establishment of homozygous knock-out sea urchins
Current Biology, i30(10):R427-R429, 2020
Miyamoto T, Hosoba K, Itabashi T, Iwane AH, Akutsu SN, Ochiai H, Saito Y, Yamamoto T and Matsuura S
Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome
The EMBO Journal, 39(12):e103499, 2020
Fujii S, Tago T, Sakamoto N, Yamamoto T, Satoh T and Satoh A
Recycling endosomes associate with Golgi stacks in sea urchin embryos
Communicative and Integrative Biology , 3(1):59-62, 2020
Iida M, Suzuki M, Sakane Y, Nishide H, Uchiyama I, Yamamoto T, Suzuki KI and Fujii S
A simple and practical workflow for genotyping of CRISPR–Cas9‐based knockout phenotypes using multiplexed amplicon sequencing
Genes to Cells, in press, 2020
Takahashi M, Ikeda K, Ohmuraya M, Nakagawa Y, Sakuma T, Yamamoto T and Kawakami K
Six1 is required for signaling center formation and labial-lingual asymmetry in developing lower incisors
Developmental Dynamics, 249(9):1098-1116, 2020
Hozumi A, Matsunobu S, Mita K, Treen N, Sugihara T, Horie T, Sakuma T, Yamamoto T, Shiraishi A, Hamada M, Satoh N, Sakurai K, Satake H and Sasakura Y
GABA-Induced GnRH Release Triggers Chordate Metamorphosis
Current Biology, 30(8), 1555-1561.e4, 2020
Ozawa K, Tsumoto H, Miura Y, Yamaguchi J, Iguchi-Ariga SMM, Sakuma T, Yamamoto T and Uchiyama Y
DJ-1 is indispensable for the S-nitrosylation of Parkin, which maintains function of mitochondria
Scientific Reports, 10, 4377, 2020
George G, Ninagawa S, Yagi H, Saito T, Ishikawa T, Sakuma T, Yamamoto T, Imami K, Ishihama Y, Kato K, Okada T and Mori K
EDEM2 stably disulfide-bonded to TXNDC11 catalyzes the first mannose trimming step in mammalian glycoprotein ERAD
Elife, 9, pii: e53455, 2020
Sawada H, Yamamoto K, Yamaguchi A, Yamada L, Higuchi A, Nukaya H, Fukuoka M, Sakuma T, Yamamoto T, Sasakura Y and Shirae-Kurabayashi M
Three multi-allelic gene pairs are responsible for self-sterility in the ascidian Ciona intestinalis
Scientific Reports, 10, 2514, 2020
Shindo R, Katagiri T, Komazawa-Sakon S, Ohmuraya M, Takeda W, Nakagawa Y, Nakagata N, Sakuma T, Yamamoto T, Nishiyama C, Nishina T, Yamazaki S, Kameda H, Nakano H
Regenerating islet-derived protein (Reg)3b plays a crucial role in attenuation of ileitis and colitis in mice
Biochem Biophys Rep, 21, 100738, 2020
Teratake Y, Takashina T, Iijima K, Sakuma T, Yamamoto T and Ishizaka Y
Development of a protein-based system for transient epigenetic repression of immune checkpoint molecule and enhancement of antitumour activity of natural killer cells
British Journal of Cancer, 122(6), 823-834, 2020
Tajima Y, Hozumi A, Yoshida K, Treen N, Sakuma T, Yamamoto T, Sasakura Y
Hox13 is essential for formation of a sensory organ at the terminal end of the sperm duct in Ciona
Developmental Biology, 458(1), 120-131, 2020
Koba H, Jin S, Imada N, Ishikawa T, Ninagawa S, Okada T, Sakuma T, Yamamoto T, Mori K
Reinvestigation of disulfide-bonded oligomeric forms of the unfolded protein response transducer ATF6
Cell Struct Funct., 45(1), 9-21, 2020
Konishi S, Tanaka N, Mashimo T, Yamamoto T, Sakuma T, Kaneko T, Tanaka M, Izawa T, Yamate J and Kuwamura M
Pathological characteristics of Ccdc85c knockout rats: a rat model of genetic hydrocephalus
Experimental Animals, 69(1), 26-33, 2020

【総説・著書】

Kunii A, Yamamoto T and Sakuma T
Various strategies of effector accumulation to improve the efficiency of genome editing and derivative methodologies
In Vitro Cellular & Developmental Biology – Animal, in press, 2020
山本 卓
ゲノム編集とはなにか
講談社ブルーバックス, 2020
山本 卓
ゲノム編集の未来
医学のあゆみ, 企画(2020)
佐久間哲史,中村志穂, 山本 卓
多様化するゲノム編集
医学のあゆみ別冊「ゲノム編集の未来」, 273:9, 700-707(2020)
落合 博
ゲノム編集ツール
医学のあゆみ別冊「ゲノム編集の未来」, 273:9, 709-715(2020)

2019年

【原著論文】

Morisaka H, Yoshimi K, Okuzaki Y, Gee P, Kunihiro Y, Sonpho E, Xu H, Sasakawa N, Naito Y, Nakada S, Yamamoto T, Sano S, Hotta A, Takeda J, Mashimo T
CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
Nat Commun., 10(1): 5302, 2019
Yasumoto S, Umemotoz, N, Lee HJ, Nakayasu M, Sawai S, Sakuma T, Yamamoto T, Mizutani M, Saito K, Muranaka T
Efficient genome engineering using Platinum TALEN in potato
Plant Biotechnology, 36: 167-173, 2019
Higuchi K, Kazeto Y, Ozaki Y, Yamaguchi T, Shimada Y, Ina Y, Soma S, Sakakura Y, Goto R, Matsubara T, Nishiki I, Iwasaki Y, Yasuike M, Nakamura Y, Matsuura A, Masuma S, Sakuma T, Yamamoto T, Masaoka T, Kobayashi T, Fujiwara A, Gen K
Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis)
Scientific Reports, 9(1):13871, 2019
Seirin-Lee S, Osakada F, Takeda J, Tashiro S, Kobayashi R, Yamamoto, Ochiai H
Role of dynamic nuclear deformation on genomic architecture reorganization
PLoS Comput Biol., 15(9):e1007289, 2019
Suenaga Y, Yamamoto M, Sakuma T, Sasada M, Fukai F, Ohira M, Yamaguchi Y, Yamamoto T, Ando K, Ozaki T, Nakagawara A.
TAp63 represses transcription of MYCN/NCYM gene and its high levels of expression are associated with favorable outcome in neuroblastoma
Biochem Biophys Res Commun, 518(2):311-318, 2019
Tanigawa S, Naganuma H, Kaku T, Era T, Sakuma T, Yamamoto, Taguchi A, Nishinakamura R
Activin Is Superior to BMP7 for Efficient Maintenance of Human iPSC-Derived Nephron Progenitors
Stem Cell Reports, 13(2):322-337, 2019
Liu D, Awazu A, Sakuma T, Yamamoto T, Sakamoto N
Establishment of knockout adult sea urchins by using a CRISPR-Cas9 system
Development Growth & Differentiation, 61(6):378-388, 2019
Li J, Dong A, Saydaminova K, Chang HZ, Wang G, Ochiai H, YamamotoT, Pertsinidis A
Single-molecule nanoscopy elucidates RNA Polymerase II transcription at single genes in live cells
Cell, 178(2):491-506.e28, 2019
Watanabe M, Nakano K, Uchikura A, Matsunari H, Yashima S, Umeyama K, Takayanagi S, Sakuma T, Yamamoto T, Morita S, Horii T, Hatada I, Nishinakamura R, Nakauchi H, Nagashima H
Anephrogenic phenotype induced by SALL1 gene knockout in pigs
Scientific Reports, 9(1):8016, 2019
Takimoto A, Kokubu C, Watanabe H, Sakuma T, Yamamoto T, Kondoh G, Hiraki Y and Shukunami C
Differential transactivation of the upstream aggrecan enhancer regulated by PAX1/9 depends on SOX9-driven transactivation
Scientific Reports, 9(1):4605, 2019
Kohara H, Utsugisawa T, Sakamoto C, Hirose L, Ogawa Y, Ogura H, Sugawara A, Aoki T, Iwasaki T, Asai T, Doisaki S, Okuno Y, Muramatsu H, Abe T, Kurita R, Miyamoto S, Sakuma T, Shiba M, Yamamoto T, Ohga S, Yoshida K, Ogawa S, Ito E, Kojima S, Kanno H, Tani K
KLF1 Mutation E325K Induces Cell-cycle Arrest in Erythroid Cells Differentiated from Congenital Dyserythropoietic Anemia (CDA) Patient-specific Induced Pluripotent Stem Cells
Experimental Hematology, 73:25-37.e8, 2019
Tsuda M, Ogawa S, Ooka M, Kobayashi K, Hirota K, Wakasugi M, Matsunaga T, Sakuma T, Yamamoto T, Chikuma S, Sasanuma H, Debatisse M, Doherty AJ, Fuchs RP, Takeda S.
PDIP38/PolDIP2 controls the DNA damage tolerance pathways by increasing the relative usage of translesion DNA synthesis over template switching
PLoS One, 14(3):e0213383, 2019
Takahashi F, Igarashi K, Takimura Y and Yamamoto T
Elucidation of secondary alcohol metabolism in Starmerella bombicola and contribution of primary alcohol oxidase FAO1
FEMS Yeast Res, 19(2). pii: foz012, 2019
Kazuki Y, Kobayashi K, Hirabayashi M, Abe S, Kajitani N, Kazuki K, Takehara S, Takiguchi M, Satoh D, Kuze J, Sakuma T, Kaneko T, Mashimo T, Osamura M, Hashimoto M, WakatsukiR, Hirashima R, Fujiwara R, Deguchi T, Kurihara A, Tsukazaki Y, Senda N, Yamamoto T, Scheer N and Oshimura M
Humanized UGT2 and CYP3A transchromosomic rats for improved prediction of human drug metabolism
Proc Natl Acad Sci U S A, 116(8):3072-3081, 2019
Nishitani A, Kunisawa N, Sugimura T, Sato K, Yoshida Y, Suzuki T, Sakuma T, Yamamoto T, Asano M, Saito Y, Ohno Y and Kuramoto T
Loss of HCN1 subunits causes absence epilepsy in rats
Brain Research, 1706:209-217, 2019
Serikawa T, Kunisawa N, Shimizu S, Kato M, Alves Iha H, Kinboshi M, Nishikawa H, Shirakawa Y, Voigt B, Nakanishi S, Kuramoto T, Kaneko T, Yamamoto T, Mashimo T, Sasa M, Ohno Y
Increased seizure sensitivity, emotional defects and cognitive impairment in PHD finger protein 24 (Phf24)-null rats
Behav Brain Res, 369:111922, 2019
Harata A, Hirakawa M, Sakuma T, Yamamoto T and Hashimoto C
Nucleotide receptor P2RY4 is required for head formation via induction and maintenance of head organizer in Xenopus laevis
Development Growth & Differentiation, 61(2):186-197, 2019

【総説・著書】

山本 卓佐久間哲史
ゲノム編集実験スタンダード
羊土社, 2019
佐久間哲史,國井厚志
ゲノム編集の意義と問題点,および解決策
腫瘍内科, 24:3, 307-314(2019)
山本 卓
ゲノム編集の基礎
ゲノム編集の新技術、細胞The CELL(ニューサイエンス社), 51(3):108-109(2019)
佐久間哲史
エフェクター集積による次世代ゲノム編集
ゲノム編集の新技術、細胞The CELL(ニューサイエンス社), 51(3):110-113(2019)
山本 卓
ゲノム編集の現状と課題
遺伝子医学、メディカルドゥ, 9(1):20-24(2019)
山本 卓
ゲノム編集の歴史と基礎
THE CHEMICAL TIMES、関東化学, 251(1):3-6(2019)
山本 卓
医療や創薬、バイオ燃料、農畜産物の品種改良や開発に世界が注目するゲノム編集(下)
科学機器, 847(2019)

2018年

【原著論文】

Kunii A, Hara Y, Takenaga M, Hattori N, Fukazawa T, Ushijima T, Yamamoto T and Sakuma T
Three-Component Repurposed Technology for Enhanced Expression (TREE): Highly Accumulable Transcriptional Activators via Branched Tag Arrays
The CRISPR Journal, 1:337-347, 2018 日刊工業新聞、中国新聞、日経バイテク
Miao W, Sakai K, Imamura R, Ito K, Suga H, Sakuma T, Yamamoto T and Matsumoto K
MET Activation by a Macrocyclic Peptide Agonist that Couples to Biological Responses Differently from HGF in a Context-Dependent Manner
Int. J. Mol. Sci., 19(10), 2018
Deguchi Y, Nishina T, Asano K, Ohmuraya M, Nakagawa Y, Nakagata N, Sakuma T, Yamamoto T, Araki K, Mikami T, Tanaka M and Nakano H.
Generation of and characterization of anti-IL-11 antibodies using newly established Il11-deficient mice
Biochem Biophys Res. Commun., 505, 453-459, 2018
Suzuki M, Hayashi T, Inoue T, Agata K, Hirayama M, Suzuki M, Shigenobu S, Takeuchi T, Yamamoto T, Suzuki KT
Cas9 ribonucleoprotein complex allows direct and rapid analysis of coding and noncoding regions of target genes in Pleurodeles waltl development and regeneration
Developmental Biology, 443, 127-136, 2018 日本経済新聞
Tanigawa S, Islam M, Sharmin S, Naganuma H, Yoshimura Y, Haque F, Era T, Nakazato H, Nakanishi K, Sakuma T, Yamamoto T, Kurihara H, Taguchi A, Nishinakamura R
Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes
Stem Cell Reports, 11, 727-740, 2018
Nakade S, Mochida K, Kinii A, Nakamae K, Aida T, Tanaka K, Sakamoto N, Sakuma T and Yamamoto T
Biased genome editing using the local accumulation of DSB repair molecules system
Nature Communications, 9, 3270, 2018 毎日新聞、科学新聞、日経産業新聞、中国新聞、日経バイオテク
Yoshida M, Yokota E, Sakuma T, Yamatsuji T, Takigawa N, Ushijima T, Yamamoto T, Fukazawa T, Naomoto Y
Development of an integrated CRISPRi targeting ΔNp63 for treatment of squamous cell carcinoma
Oncotarget, 9, 29220-29232, 2018 朝日新聞、日経産業新聞、中国新聞、山陽新聞、週刊新潮、デイリー新潮
Nakagawa Y, Sakuma T, Takeo T, Nakagata N and Yamamoto T
Electroporation-mediated genome editing in vitrified/warmed mouse zygotes created by IVF via ultra-superovulation
Experimental Animals, 67, 535-543, 2018
Saha LK, Kim S, Kang H, Akter S, Choi K, Sakuma T, Yamamoto T, Sasanuma H, Hirota K, Nakamura J, Honma M, Takeda S and Dertinger S
Differential micronucleus frequency in isogenic human cells deficient in DNA repair pathways is a valuable indicator for evaluating genotoxic agents and their genotoxic mechanisms
Environmental and Molecular Mutagenesis, 59, 529-538, 2018
Takashina T, Koyama T, Nohara S, Hasegawa M, Ishiguro A, Iijima K, Jun L, Shimura M, Okamura T, Sakuma T, Yamamoto T and Ishizaka Y
Identification of a cell-penetrating peptide applicable to a protein-based transcription activator-like effector expression system for cell engineering
Biomaterials, 173:11-21, 2018 日経産業新聞
Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, Ohishi M, Sakuma T, Soga T, Yamamoto T and Woltjen K
Microhomology-assisted scarless genome editing in human iPSCs
Nature Communications, 9, 939, 2018 日本経済新聞、日刊工業新聞、中国新聞、化学
Shukunami C, Takimoto A, Nishizaki Y, Yoshimoto Y, Tanaka S, Miura S, Watanabe H, Sakuma T, Yamamoto T, Kondoh G, Hiraki Y
Scleraxis is a transcriptional activator that regulates the expression of Tenomodulin, a marker of mature tenocytes and ligamentocytes
Scientific Reports, 8:3155, 2018
Tanaka Y, Sone T, Higurashi N, Sakuma T, Suzuki S, Ishikawa M, Yamamoto T, Mitsui J, Tsuji H, Okano H and Hirose S
Generation of D1-1 TALEN isogenic control cell line from Dravet syndrome patient iPSCs using TALEN-mediated editing of the SCN1A gene
Stem Cell Research, 28:100-104, 2018
Kuriyama S, Tsuji T, Sakuma T, Yamamoto T and Tanaka M
PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetro-oligomerization through interaction with Bid in human colon cancer
Cell Death Discovery, 4, 11, 2018
Okamoto Y, Iwasaki WM, Kugou K, Takahashi KK, Oda A, Sato K, Kobayashi W, Kawai H, Sakasai R, Takaori-Kondo A, Yamamoto T, Kanemaki MT, Taoka M, Isobe T, Kurumizaka H, Innan H, Ohta K, Ishiai M and Takata M.
Replication stress induces accumulation of FANCD2 at central region of large fragile genes
Nucleic Acid Research, 46(6):2932-2944, 2018
Kinjo S, Kiyomoto M, Yamamoto T, Ikeo K and Yaguchi S
HpBase: a genome database of a sea urchin, Hemicentrotus pulcherrimus
Development Growth & Differentiation, 60(3):174-182, 2018
Sakuma T, Mochida K, Nakade S, Ezure T, Minagawa S and Yamamoto T
Unexpected heterogeneity derived from Cas9 ribonucleoprotein-introduced clonal cells at the HPRT1 locus
Genes to Cells, 23(4):255-263, 2018
Sato’o Y, Hisatsune J, Yu L, Sakuma T, Yamamoto T and Sugai M.
Tailor-made gene silencing of Staphylococcus aureus clinical isolates by CRISPR interference.
PLoS One, 13(1):e0185987, 2018
Kawabe Y, Komatsu S, Komatsu S, Murakami M, Ito A, Sakuma T, Nakamura T, Yamamoto T and Kamihira M
Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems
Journal of Bioscience and Bioengineering, 125(5):599-605, 2018
Sakane Y, Iida M, Hasebe T, Fujii S, Buchholz DR, Ishizuya-Oka A, Yamamoto T and Suzuki KT
Functional analysis of thyroid hormone receptor beta in Xenopus tropicalis founders using CRISPR-Cas
Biol Open, 7(1). pii: bio030338, 2018

【総説・著書】

Sakuma T, Yamamoto T
Acceleration of cancer science with genome editing and related technologies
Cancer Science, 109(12):3679-3685, 2018
Suzuki KT, Sakane Y, Suzuki M, Yamamoto T
A Simple Knock-In System for Xenopus via Microhomology Mediated End Joining Repair
Methods Mol Biol, 1865, 91-103, 2018
Miyamoto T, Akutsu SN, Tauchi H, Kudo Y, Tashiro S, Yamamoto T, Matsuura S
Exploration of genetic basis underlying individual differences in radiosensitivity within human populations using genome editing technology
Journal of Radiation Research, 59(suppl_2):ii75-ii82, 2018
Sakuma T, Yamamoto T
Genome editing for dissecting and curing human genetic diseases
Journal of Human Genetics, 63, 105, 2018
Nakade A, Yamamoto T and Sakuma T
Cancer induction and suppression with transcriptional control and epigenome editing technology
Journal of Human Genetics, 63, 187–194, 2018
山本 卓
ゲノム編集の基本原理
生物の科学「遺伝」, 72:6, 566-571(2018)
山本 卓
ゲノム編集に用いるヌクレアーゼとノックイン技術
血液フロンティア、医薬ジャーナル社, 28:6, 1017-1022(2018)
佐久間哲史, 山本 卓
総論:ゲノム編集
医療応用をめざすゲノム編集、化学同人, 16-27(2018)
山本 卓
ゲノム編集
メディカル・サイエンス・ダイジェスト、ニューサイエンス社, 2, 398-400(2018)
山本 卓
ゲノム編集のもたらすブレイクスルー
ファルマシア, 54:p101(2018)
山本 卓
医療や創薬、バイオ燃料、農畜産物の品種改良や開発に世界が注目するゲノム編集(上)
科学機器, 846(2018)

【教科書】

山本 卓
ゲノム編集の基本原理と応用
裳華房, 2018

2017年

【原著論文】

Tsuda M, Cho K, Ooka M, Shimizu N, Watanabe R, Yasui A, Nakazawa Y, Ogi T, Harada H, Agama K, Nakamura J, Asada R, Fujiike H, Sakuma T, Yamamoto T, Murai J, Hiraoka M, Koike K, Pommier Y, Takeda S and Hirota K
ALC1/CHD1L, a chromatin-remodeling enzyme, is required for efficient base excision repair
PLoS One, 12, e0188320, 2017
Matsushita M, Ochiai H, Suzuki KT, Hayashi S, Yamamoto T, Awazu A and Sakamoto N
Dynamic changes in the interchromosomal interaction of early histone gene loci during early development of sea urchin
Journal of Cell Science, 130(24):4097-4107, 2017
Abe S, Kobayashi K, Oji A, Sakuma T, Kazuki K, Takehara S, Nakamura K, Okada A, Tsukazaki Y, Senda N, Honma K, Yamamoto T, Ikawa M, Chiba K, Oshimura M and Kazuki Y
Modification of single-nucleotide polymorphism in a fully humanized CYP3A mouse by genome editing technology
Scientific Reports, 7, 15189, 2017
Matsuzaki Y, Sakuma T, Yamamoto T and Saya H
Establishment of pten knockout medaka with transcription activator-like effector nucleases (TALENs) as a model of PTEN deficiency disease
PLoS One, 12(10):e0186878, 2017
Miyamoto T, Natsuko Akutsu S, Fukumitsu A, Morino H, Masatsuna T, Hosoba K, Kawakami H, Yamamoto T, Shimizu K, Ohashi H and Matsuura S
PLK1-mediated phosphorylation of WDR62/MCPH2 ensures proper mitotic spindle orientation
Human Molecular Genetics, 26(22):4429-4440, 2017
Nakamura M, Kurungsee T, Sakuma T, Yamamoto T and Yanaka N
TALEN-mediated targeted editing of the GDE5 gene suppresses fibroblastic cell proliferation
Biosci Biotechnol Biochem, 81(11):2164-2167, 2017
Mori J, Sanoh S, Kashiwagi K, Hanada H, Shigeta M, Suzuki KT, Yamamoto T, Kotake Y, Sugihara K, Kitamura S, Kashiwagi A and Ohta S
Developmental changes in drug-metabolizing enzyme expression during metamorphosis of Xenopus tropicalis
The Journal of Toxicological Sciences, 42(5):605-613, 2017
Royba E, Miyamoto T, Akutsu S, Hosoba K, Tauchi H, Kudo Y, Tashiro S, Yamamoto T and Matsuura S
Evaluation of ATM heterozygous mutations underlying individual differences in radiosensitivity using genome editing in human cultured cells
Scientific Reports, 7, 5996, 2017
Kaku Y, Taguchi A, Tanigawa S, Haque F, Sakuma T, Yamamoto T and Nishinakamura R
PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells
Scientific Reports, 7, 4554, 2017
Oike A, Kodama M, Yasumasu S, Yamamoto T, Nakamura Y, Ito E and Nakamura M
Participation of androgen and its receptor in sex determination of an amphibian species
PLoS One, 12(6):e0178067, 2017
Nakagawa Y, Sakuma T, Nishimichi N, Yokosaki Y, Takeo T, Nakagata N and Yamamoto T
Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice
Biology Open, 6(5):706-713, 2017
Nakamae K, Nishimura Y, Takenaga M, Nakade S, Sakamoto N, Ide H, Sakuma T and Yamamoto T
Establishment of expanded and streamlined pipeline of PITCh knock-in – a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO
Bioengineered, 8(3):302-308, 2017
Yoshida K, Nakahata A, Treen N, Sakuma T, Yamamoto T and Sasakura Y
Hox-mediated endodermal identity patterns the pharyngeal muscle formation in the chordate pharynx
Development, 144(9):1629-1634, 2017
Kameda T, Isami S, Togashi Y, Nishimori H, Sakamoto N and Awazu A
The 1-Particle-per-k-Nucleotides (1PkN) Elastic Network Model of DNA Dynamics with Sequence-Dependent Geometry
Front Physiol, 8:103, 2017
Takayama K, Igai K, Hagihara Y, Hashimoto R, Hanawa M, Sakuma T, Tachibana M, Sakurai F, Yamamoto T and Mizuguchi H
Highly efficient biallelic genome editing of human ES/iPS cells Using a CRISPR/Cas9 or TALEN system
Nucleic Acid Research, 45(9):5198-5207, 2017
Mizutani O, Arazoe T, Toshida K, Hayashi R, Ohsato S, Sakuma T, Yamamoto T, Kuwata S and Yamada O
Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant
Journal of Bioscience and Bioengineering, 123:287-293, 2017
Yoshida K, Hozumi A, Treen N, Sakuma T, Yamamoto T, Shirae-Kurabayashi M and Sasakura Y
Germ cell regeneration-mediated, enhanced mutagenesis in the ascidian Ciona intestinalis
reveals flexible germ cell formation from different somatic cells
Developmental Biology, 423:111-125, 2017

【総説・著書】

Sakuma T and Yamamoto T
Magic wands of CRISPR – lots of choices for gene knock-in
Cell Biology & Toxicology, 33:501-505, 2017
Takata N, Sakakura E, Sakuma T and Yamamoto T
Genetic Tools for Self-Organizing Culture of Mouse Embryonic Stem Cells via Small Regulatory RNA-Mediated Technologies, CRISPR/Cas9, and Inducible RNAi
RNAi and Small Regulatory RNAs in Stem Cells, Methods Mol Biol., 1622:269-292
Sakuma T and Yamamoto T
Current overview of TALEN construction systems
Genome Editing in Animals, Methods Mol Biol., 1630:25-36
Sakane Y, Suzuki KT and Yamamoto T
A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis founders using the CRISPR-Cas System
Genome Editing in Animals, Methods Mol Biol., 1630:189-203
Ochiai H and Yamamoto T
Construction and Evaluation of Zinc Finger Nucleases
Genome Editing in Animals, Methods Mol Biol., 1630:1-24
Nakade S, Yamamoto T and Sakuma T
Cas9, Cpf1 and C2c1/2/3―What’s next?
Bioengineered, 8:265-273, 2017
Sakuma T, Sakamoto T and Yamamoto T
All-in-One CRISPR-Cas9/FokI-dCas9 Vector-Mediated Multiplex Genome Engineering in Cultured Cells
In Vitro Mutagenesis, Methods Mol Biol., 1498:41-56, 2017
佐久間哲史
ゲノム編集の最新方法論
DNA鑑定, 9:1-8(2017)
佐久間哲史
医学分野におけるゲノム編集の現状と将来展望
CLINICAL CALCIUM, 27:1788-1793(2017)
山本 卓
ゲノム編集の基本原理
CLINICAL CALCIUM, 27:1638-1644(2017)
佐久間哲史中出翔太山本 卓
エピゲノム編集
実験医学別冊「エピジェネティクス実験スタンダード」(牛島俊和, 眞貝洋一, 塩見春彦編), 羊土社, pp345-352(2017)
佐久間哲史、高田 望
ゲノム編集の最新動向
ナノバイオ・メディシン-細胞核内反応とゲノム編集(宇理須恒雄編), 近代科学社, pp7-12 (2017)
佐久間哲史
ゲノム編集の歴史と現状
ナノバイオ・メディシン-細胞核内反応とゲノム編集(宇理須恒雄編), 近代科学社, pp135-150 (2017)
山本 卓
「CRISPR-Cas9」とはどのような技術か?
実験医学, 35:2199, 2017
山本 卓
ゲノム編集技術の進展
日本臨床, 75:778-782, 2017
佐久間哲史
進展するゲノム編集
現代化学, 551:18-22, 2017
山本 卓
ゲノム編集とはどんな技術なのか
日本化学会バイオテクノロジー部会ニュースレター, 20(2):3-6, 2017

2016年

【原著論文】

Aida T, Nakade S, Sakuma T, Izu Y, Oishi A, Mochida K, Ishikubo H, Usami T, Aizawa H, Yamamoto T and Tanaka K
Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ
BMC Genomics, 17, 979, 2016
Toyonaga K, Torigoe S, Motomura Y, Kamichi T, Hayashi JM, Morita YS, Noguchi N, Chuma Y, Kiyohara H, Matsuo K, Tanaka H, Nakagawa Y, Sakuma T, Ohmuraya M, Yamamoto T, Umemura M, Matsuzaki G, Yoshikai Y, Yano I, Miyamoto T, Yamasaki S
C-Type Lectin Receptor DCAR Recognizes Mycobacterial Phosphatidyl-Inositol Mannosides to Promote a Th1 Response during Infection
Immunity, 45(6): 1245-1257, 2016
Sasakura Y, Ogura Y, Treen N, Yokomori R, Park S-J, Nakai K, Saiga H, Sakuma T, Yamamoto T, Fujiwara S, Yoshida K
Transcriptional regulation of a horizontally transferred gene from bacterium to chordate
Proceedings B, 283(1845), 2016
Tochio N, Umehara K, Uewaki J, Flechsig H, Kondo M, Dewa T, Sakuma T, Yamamoto T, Saitoh T, Togashi Y and Tate SI
Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy
Sci Rep, 6, 37887, 2016
Suzuki KT, Suzuki M, Shigeta M, Fortriede JD, Takahashi S, Mawaribuchi S, Yamamoto T, Taira M and Fukui A
Clustered Xenopus keratin genes: A genomic, transcriptomic, and proteomic analysis
Developmental Biology, 426(2):384-392
Sakuma T, Masaki K, Abe-Chayama H, Mochida K, Yamamoto T and Chayama K
Highly multiplexed CRISPR-Cas9-nuclease and Cas9-nickase vectors for inactivation of hepatitis B virus
Genes to Cells, 21, 1253-1262, 2016
Sato K, Oiwa R, Kumita W, Henry R, Sakuma T, Ito R, Nozu R, Inoue T, Katano I, Sato K, Okahara N, Okahara J, Yamamoto M, Hanazawa K, Kawakami T, Kametani Y, Suzuki R, Takahashi T, Weinstein E, Yamamoto T, Sakakibara Y, Habu S, Hata J, Okano H and Sasaki E
Non-human primate model of severe combined immunodeficiency using highly efficient genome editing
Cell Stem Cell, 19, 127-138, 2016
Yabe T, Hoshijima K, Yamamoto T and Takada S
Mesp quadruple zebrafish mutant reveals different roles of mesp genes in somite segmentation between mouse and zebrafish
Development, 143, 2842-2852, 2016
Sasaki T, Hanisch F-G, Deutzmann R, Sakai LY, Sakuma T, Miyamoto T, Yamamoto T, Hannappel E, Chu M-L, Lanig H and von der Mark K
Functional consequence of fibulin-4 missense mutations associated with vascular abnormalities and cutis laxa
Matrix Biology, 56, 132-149, 2016
Nakagawa Y, Sakuma T, Nishimichi N, Yokosaki Y, Yanaka N, Takeo T, Nakagata N, and Yamamoto T
Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout,single-amino-acid-substituted, and floxed mice
Biol Open, 5, 1142-1148, 2016
Nii T, Kohara H, Marumoto T, Sakuma T, Yamamoto T and Tani K
Single-Cell-State Culture of Human Pluripotent Stem Cells Increases Transfection Efficiency
Biores Open Access, 5, 127-136, 2016
Shigeta M, Sakane Y, Iida M, Suzuki M, Kashiwagi K, Kashiwagi A, Fujii S, Yamamoto T and Suzuki KT
Rapid and efficient analysis of gene function using CRISPR-Cas9 in Xenopus tropicalis founders
Genes to Cells, 21, 755-771, 2016
Ichiyanagi N, Fujimori K, Yano M, Ishihara-Fujisaki C, Sone T, Akiyama T, Okada Y, Akamatsu W, Matsumoto T, Ishikawa M, Nishimoto Y, Ishihara Y, Sakuma T, Yamamoto T, Tsuiji H, Suzuki N, Warita H, Aoki M and Okano H
Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells
Stem Cell Reports, 6, 496-510, 2016
Nishitani A, Tanaka M, Shimizu S, Yokoe M, Yoshida Y, Suzuki T, Sakuma T, Yamamoto T, Kuwamura M, Takemoto S, Ohono Y and Kuramoto T
Involvement of aspartoacylase in tremor expression in rats
Experimental Animals, 65, 293-301, 2016
Banno K, Omori S, Hirata K, Nawa N, Nakagawa N, Nishimura K, Ohtaka M, Nakanishi M, Sakuma T, Yamamoto T, Toki T, Ito E, Yamamoto T, Kokubu C, Takeda J, Taniguchi H, Arahori H, Wada K, Kitabatake Y and Ozono K
Systematic Cellular Disease Models Reveal Synergistic Interaction of Trisomy 21 and GATA1 Mutations in Hematopoietic Abnormalities
Cell Reports, 15, 1228-1241, 2016
Woltjen K, Yamamoto T, Kokubu C and Takeda J
Report on the Conference on Transposition and Genome Engineering 2015 (TGE 2015): Advancing cutting-edge genomics technology in the ancient city of Nara
Genes to Cells, 21, 392-395, 2016
Takemoto A, Miyamoto T, Simono F, Kurogi N, Shirae-Kurabayashi M, Awazu A, Suzuki KT, Yamamoto T and Sakamoto N
Cilia play a role in breaking left–right symmetry of the sea urchin embryo
Genes to Cells, 21, 568-578, 2016
Nakahara Y, Muto A, Hirabayashi R, Sakuma T, Yamamoto T, Kume S and Kikuchi Y
Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish
Genes to Cells, 21, 492-504, 2016
Marsan E, Ishida S, Schramm A, Weckhuysen S, Muraca G, Lecas S, Liang N, Treins C, Pende M, Roussel D, Quyen MLV, Mashimo T, Kaneko T, Yamamoto T, Sakuma T, Mahon S, Miles R, Leguern E, Charpier S and Baulac S
Depdc5 knockout rat: a novel model of mTORopathy
Neurobiology of Disease, 89, 180-189, 2016
Takata N, Sakakura E, Kasukawa T, Sakuma T, Yamamoto T and Sasai Y
Establishment of functional genomics pipeline in epiblast-like tissue by combining transcriptomic analysis and gene knock-down/-in/-out using RNAi and CRISPR/Cas9
Human Gene Therapy, 27, 436-450, 2016
Guo L, Yamashita H, Kou I, Takimoto A, Meguro-Horike M, Sakuma T, Miura S, Adachi T, Yamamoto T, Ikegawa S, Hiraki Y and Shukunami C
Functional investigation of a non-coding variant associated with adolescent idiopathic scoliosis in zebrafish: elevated expression of the ladybird homeobox gene causes body axis deformation
PLoS Genetics, 12, e1005802, 2016 日経バイオテクonline
Suzuki M, Takagi C, Miura S, Sakane Y, Suzuki M, Sakuma T, Sakamoto N, Endo T, Kamei Y, Sato Y, Kimura H, Yamamoto T, Ueno N and Suzuk KT
In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration
Genes to Cells, 21, 358-369, 2016 日経バイオテクonline
Sakuma T, Nakade S, Sakane Y, Suzuki KT and Yamamoto T
MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems
Nature Protocols, 11, 118–133, 2016 日経バイオテクonline
Sharmin S, Taguchi A, Kaku Y, Yoshimura Y, Ohmori T, Sakuma T, Mukoyama M, Yamamoto T, Kurihara H and Nishinakamura R
Human iPS cell-derived podocytes expressing typical molecular signatures maturate upon transplantation
Journal of the American Society of Nephrology, 27, 1778-1791, 2016 毎日新聞
Rao S, Fujimura T, Matsunari H, Sakuma T, Nakano K, Watanabe M, Asano Y, Kitagawa E, Yamamoto T and Nagashima H.
Efficient modification of myostatin gene in porcine somatic cells and generation of knockout piglets.
Molecular Reproduction and Development, 83:61–70, 2016 読売新聞、Yahooニュース

【総説・著書】

山本 卓
ゲノム編集の基本原理
ゲノム編集入門(山本 卓編), 裳華房(2016)
坂本尚昭
海産無脊椎動物でのゲノム編集の利用
ゲノム編集入門(山本 卓編), 裳華房(2016)
鈴木賢一
両生類でのゲノム編集の利用
ゲノム編集入門(山本 卓編), 裳華房(2016)
佐久間哲史中出翔太
新規ゲノム編集ツールの開発動向
実験医学別冊「All Aboutゲノム編集」(真下知士、山本 卓編), 羊土社(2016)
佐久間哲史中出翔太山本 卓
さまざまな遺伝子ノックインシステム
実験医学別冊「All Aboutゲノム編集」(真下知士、山本 卓編), 羊土社(2016)
鈴木賢一
両生類のゲノム編集
実験医学別冊「All Aboutゲノム編集」(真下知士、山本 卓編), 羊土社(2016)
坂本尚昭山本 卓
左右相称のプルテウス幼生から五放射相称のウニへ
生物科学, 67:133-138, 2016
Sakuma T and Yamamoto T
Engineering Customized TALENs Using the Platinum Gate TALEN Kit
TALENs: Methods and Protocols, Methods Mol Biol., 1338:61-70, 2016
山本 卓坂本尚昭
ゲノム編集の基礎と応用
臨床血液, 57:1869-1873, 2016
山本 卓
ゲノム編集技術の原理と応用
大阪大学医学部学友会会誌, 36:65-68, 2016

【教科書】

山本 卓
動物のからだ作り
基礎生物科学(高橋純夫編), 培風館, pp84-91(2016)
坂本尚昭
DNAの構造と複製、
基礎生物科学(高橋純夫編), 培風館, pp67-74(2016)

【特許】

佐久間哲史山本 卓
植物細胞へのタンパク質の導入法(理化学研究所、宇都宮大学、筑波大学との共同出願)、特願2016-009207 (2016)

 

2015年

【原著論文】

Ninagawa S, Okada T, Sumitomo Y, Horimoto S, Sugimoto T, Ishikawa T, Takeda S, Yamamoto T, Suzuki T, Kamiya Y, Kato K and Mori K
Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway
Journal of Cell Biology, 211: 775-784, 2015
Hoa NN, Akagawa R, Yamasaki T, Hirota K, Sasa K, Natsume T, Kobayashi J, Sakuma T, Yamamoto T, Komatsu K, Kanemaki M, Pommier Y, Takeda S and Sasanuma H
Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11 to the initial step of double-strand break repair by homologous DNA recombination in the both chicken DT40 and human TK6 cell lines
Genes to Cells, 20:1059–1076, 2015
Isami S, Sakamoto N, Nishimori H and Awazu A
Simple Elastic Network Models for Exhaustive Analysis of Long Double-Stranded DNA Dynamics with Sequence Geometry Dependence
PLoS One, 211(4): 775-784, 2015
Miyamoto K, Suzuki K, Suzuki M, Sakane Y, Sakuma T, Herberg S, Simeone A, Simpson D, Jullien J, Yamamoto T and Gurdon JB
The Expression of TALEN before Fertilization Provides a Rapid Knock-out Phenotype in Xenopus laevis Founder Embryos
PLoS One, 10(11):e0142946, 2015 読売新聞、日経バイオテクonline
Sakuma T, Takenaga M, Kawabe Y, Nakamura T, Kamihira M and Yamamoto T
Homologous recombination-independent large gene cassette knock-in in CHO cells using TALEN and MMEJ-directed donor plasmid
International Journal of Molecular Sciences, 16: 23849-23866, 2015
Sasaki K, Yokobayashi S, Nakamura T, Okamoto I, Yabuta Y, Kurimoto K, Ohta H, Moritoki Y, Iwatani C, Tsuchiya H, Nakamura S, Sekiguchi K, Sakuma T, Yamamoto T, Mori T, Woltjen K, Nakagawa M, Yamamoto T, Takahashi K, Yamanaka S and Saitou M
Robust in vitro induction of human germ cell fate from pluripotent stem cells.
Cell Stem Cell, 17: 178-194, 2015
Ochiai H, Sugawara T and Yamamoto T
Simultaneous live imaging of the transcription and nuclear position of specific genes.
Nucleic Acid Research, doi: 10.1093/nar/gkv624, 2015 日刊工業新聞、Eurekalert!、Alphagalileo、日経バイオテクonline
Keka I, Mohiuddin M, Rahman MM, Maede Y, Sakuma T, Honma M, Yamamoto T, Takeda S and Sasanuma H
Smarcal1 Promotes Double-Strand-Break Repair by Nonhomologous End-Joining
Nucleic Acid Research, 43: 6359-6372, 2015 日経バイオテクonline
Iizuka H, Kagoya Y, Kataoka K, Yoshimi A, Miyauchi M, Taoka K, Kumano K, Yamamoto T, Hotta A, Arai S and Kurokawa M.
Targeted gene correction of RUNX1 in induced pluripotent stem cells derived from familial platelet disorder with propensity to myeloid malignancy restores normal megakaryopoiesis.
Experimental Hematology, 43: 849-857, 2015
Fujiwara M, Fujimura K, Obata S, Yanagibashi R, Sakuma T, Yamamoto T and Suzuki ST
Epithelial DLD-1 cells with disrupted E-cadherin gene retain the ability to form cell junctions
Cell Structure and Function, 40: 79-94, 2015
Sato T, Sakuma T, Yokonishi T, Katagiri K, Ogonuki N, Ogura A, Yamamoto T and Ogawa T
Genome editing in mouse spermatogonial stem cell lines using TALEN and double-nicking CRISPR/Cas9 systems
Stem Cell Reports, 5: 75-82, 2015
Fujiwara M, Nagatomo A, Tsuda M, Obata S, Sakuma T, Yamamoto T and Suzuki ST
Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring the juxtamembrane region and plakophilins
Journal of Biochemistry, 158: 339-353
Hara S, Tamano M, Yamashita S, Kato T, Saito T, Sakuma T, Yamamoto T, Inui M and Takada S
Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9
Scientific Reports, 5: 11221, 2015
Nakagawa Y, Sakuma T, Sakamoto T, Ohmuraya M, Nakagata N and Yamamoto T
Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes
BMC Biotechnology, 15: 33, 2015
Aida T, Chiyo K, Usami T, Ishikubo H, Imahashi R, Wada Y, Tanaka K, Sakuma T, Yamamoto T and Tanaka K
Cloning-free CRISPR/Cas system facilitates functional cassette knockin in mice
Genome Biology, 16: 87, 2015 日刊工業新聞、Genome BiologyのReseach Highlight、日経バイオテクonline
Nakade S, Sakuma T, Sakane Y, Hara Y, Kurabayashi A, Kashiwagi K, Kashiwagi A, Yamamoto T and Obara M
Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs
In Vitro Cellular & Developmental Biology – Animal, 51: 879-884, 2015
Kawai N, Ogura Y, Ikuta T, Saiga H, Hamada M, Sakuma T, Yamamoto T, Satoh N and Sasakura Y
Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine
Developmental Biology, 403: 43-56, 2015
Uemura N, Koike M, Ansai S, Kinoshita M, Ishikawa-Fujiwara T, Matsui H, Naruse K, Sakamoto N, Uchiyama Y, Todo T, Takeda S, Yamakado H and Takahashi R
Viable neuronopathic Gaucher disease model in Medaka (Oryzias latipes) displays axonal accumulation of alpha-synuclein
PLoS Genetics, 11: e1005065, 2015
Ebina H, Kanemura Y, Misawa N, Sakuma T, Kobayashi T, Yamamoto T and Koyanagi Y
A high excision potential of TALENs for integrated DNA of HIV-based lentiviral vector
PLoS One, 10: e0120047, 2015
Hisano Y, Sakuma T, Nakade S, Ohga R, Ota S, Okamoto H, Yamamoto T and Kawahara A
Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish
Scientific Reports, 5: 8841, 2015 朝日新聞、日刊工業新聞、山梨日日新聞、日経バイオテクonline、Nature Japan注目の論文
Arazoe T, Ogawa T, Miyoshi K, Yamato T, Ohsato S, Sakuma T, Yamamoto T, Arie T and Kuwata S
Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus
Biotechnology and Bioengineering, 112: 1335-1342, 2015 日経バイオテクonline
Miyamoto T, Hosoba K, Ochiai H, Royba E, Izumi H, Sakuma T, Yamamoto T, Dynlacht BD and Matsuura S
The microtubule depolymerizing activity of a mitotic kinesin protein KIF2A drives primary cilia disassembly coupled with cell proliferation
Cell Reports, doi:10.1016/j.celrep.2015.01.003, 2015
Choi J, Suzuki KT, Sakuma T, Shewade L, Yamamoto T and Buchholz DR
Unliganded thyroid hormone receptor alpha regulates developmental timing via gene repression as revealed by gene disruption in Xenopus tropicalis
Endocrinology, 156: 735-744, 2015 EndocrinologyのNews and Views、Cell & BioscienceのCommentaryにピックアップ
Li HL, Fujimoto N, Sasakawa N, Shirai S, Ohkame T, Sakuma T, Tanaka M, Amano N, Watabnabe A, Sakurai H, Yamamoto T, Yamanaka S and Hotta A
Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9
Stem Cell Reports, 4: 143-154, 2015 朝日新聞、読売新聞、毎日新聞、共同通信より配信、日経バイオテクonline
Sakuma T
Front-line of genome editing technology for animal cell engineering
BMC Proceedings, 9(Suppl 9): O1, 2015
Okada J, Kikuta S, Gusev O, Suetsugu Y, Cornette R, Sakuma T, Yamamoto T and Kikawada T
Construction of Optimized CRISPR/Cas System to Reveal the Mechanisms of Anhydrobiosis in the Sleeping Chironomid
Cryobiology and Cryotechnology, 61: 69-73, 2015

【総説・著書】

Ochiai H and Yamamoto T
Genome editing using zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)
Targeted Genome Editing Using Site-Specific Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System (Ed by Yamamoto T), Springer, pp3-24 (2015)
Sakuma T and Yamamoto T
CRISPR/Cas9: The Leading Edge of Genome Editing Technology
Targeted Genome Editing Using Site-Specific Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System (Ed by Yamamoto T), Springer, pp25-41 (2015)
Sakamoto N
Genome editing in sea urchin
Targeted Genome Editing Using Site-Specific Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System (Ed by Yamamoto T), Springer, pp97-106 (2015)
Suzuki KT and Hayashi T
Genome Editing Using Site-Specific Nucleases in Amphibians
Targeted Genome Editing Using Site-Specific Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System (Ed by Yamamoto T), Springer, pp133-149 (2015)
山本 卓佐久間哲史鈴木賢一
論文だけではわからない ゲノム編集成功の秘訣Q&A (2015)
中出翔太佐久間哲史
ゲノム編集、ホルモンと臨床、Vol.62(2)、p67-72 (2015)
山本 卓佐久間哲史坂本尚昭
ZFN, TALEN, CRISPR/Cas9システムとは、進化するゲノム編集技術、p11-14 (2015)
佐久間哲史山本 卓
相同組換えに依存しない簡便・正確・高効率な遺伝子ノックイン法:PITChシステム、進化するゲノム編集技術、p59-66 (2015)
坂根祐人山本 卓鈴木賢一
ツメガエルにおけるゲノム編集、進化するゲノム編集技術、p179-187 (2015)
高田 望、佐久間哲史
ゲノム編集の基礎と応用:ゲノム編集技術と立体培養技術の融合を例に、ナノ学会会報、Vol.13(2)、p79-84 (2015)
佐久間哲史山本 卓
“all-in-one”CRISPR/Cas9ベクターシステムを用いた多重ゲノム編集、実験医学、Vol.33(6)、p959-965 (2015)
山本 卓坂本尚昭佐久間哲史
ゲノム編集技術の現状と展望、再生医療、Vol.14(1)、p34-40 (2015)
佐久間哲史
Platinum TALENおよびCRISPR/Cas9を用いたゲノム編集、比較内分泌学、Vol.41(154)、p11-13 (2015)
佐久間哲史山本 卓
ゲノム編集の基礎、医学のあゆみ、Vol.252(2)、p147-151 (2015)

【特許】

落合 博、山本 卓
細胞の作製方法および該作製方法で作製された細胞、特願2015-080648 (2015)

 

2014年

【原著論文】

Nakade S*, Tsubota T*, Sakane Y*, Kume S, Sakamoto N, Obara M, Daimon T, Sezutsu H, Yamamoto T, Sakuma T# and Suzuki K#
Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9
Nature Communications, 5: 5560, 2014 日本経済新聞、中国新聞、共同通信より配信、日経バイオテクonline、Nature Japan注目の論文
Ochiai H, Sugawara T, Sakuma T and Yamamoto T
Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells
Scientific Reports, 4: 7125, 2014 Nature Japan注目の論文
Hiruta C, Ogino Y, Sakuma T, Toyota K, Miyagawa S, Yamamoto T and Iguchi T
Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex
BMC Biotechnology, 14: 95, 2014
Kaneko T, Sakuma T, Yamamoto T and Mashimo T
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
Scientific Reports, 4: 6382, 2014 日刊工業新聞、日経バイオテクonline、Nature Japan注目の論文、BioTechnique誌
Sawai S, Ohyama K, Yasumoto S, Seki H, Sakuma T, Yamamoto T, Takebayashi Y, Kojima M, Sakakibara H, Aoki T, Muranaka T, Saito K and Umemoto N.
Sterol Side Chain Reductase 2 Is a Key Enzyme in the Biosynthesis of Cholesterol, the Common Precursor of Toxic Steroidal Glycoalkaloids in Potato
Plant Cell, 26: 3763-3774, 2014 日経バイオテクonline、時事通信より配信
Kazuki Y, Yakura Y, Abe S, Osaki M, Kajitani N, Kazuki K, Takehara S, Honma K, Suemori H, Yamazaki S, Sakuma T, Toki T, Shimizu R, Nakauchi H, Yamamoto T and Oshimura M
Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies
Scientific Reports, 4: 6136, 2014 日本海新聞
Ninagawa S, Okada T, Sumitomo Y, Kamiya Y, Kato K, Horimoto S, Ishikawa T, Takeda S, Sakuma T, Yamamoto T and Mori K
EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step
Journal of Cell Biology, 206: 347-356, 2014 F1000Prime Recommended、京都新聞、中日新聞、科学新聞、日経バイオテクonline
Nakagawa Y*, Sakuma T*, Nakagata N, Yamasaki S, Takeda N, Ohmuraya M and Yamamoto T
Application of oocyte cryopreservation technology in TALEN-mediated mouse genome editing
 Experimental Animals,63: 349-355, 2014
Yasue A, Mitsui SN, Watanabe T, Sakuma T, Oyadomari S, Yamamoto T, Noji S, Mito T and Tanaka E
Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems
Scientific Reports, 4: 5705, 2014
Sakuma T, Nishikawa A, Kume S, Chayama K and Yamamoto T
Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system
Scientific Reports, 4: 5400, 2014 Nature Japan注目の論文
Yoshida K, Treen N, Hozumi A, Sakuma T, Yamamoto T and Sasakura Y
Germ cell mutations of the ascidian Ciona intestinalis with TALE nucleases
Genesis, 52: 431-439, 2014
Tokumasu D*, Sakuma T*, Hayashi Y, Hosoi S, Hiyama E and Yamamoto T
FAST-id system for enrichment of cells with TALEN-induced mutations and large deletions
Genes to Cells, 19: 419-431, 2014
Ochiai H*, Miyamoto T*, Kanai A, Hosoba K, Sakuma T, Kudo Y, Asami K, Ogawa A, Watanabe A, Kajii T, Yamamoto T and Matsuura S
TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS (MVA) syndrome
Proc Natl Acad Sci U S A, 111: 1461-1466, 2014 PNAS Commentaryにピックアップ
Treen N, Yoshida K, Sakuma T, Sasaki H, Kawai N, Yamamoto T and Sasakura Y
Tissue-specific and ubiquitous gene knockouts in Ciona by electroporating TALENs provide new approaches to investigate gene functions
Development, 141: 481-487, 2014
Sugi T*, Sakuma T*, Ohtani T and Yamamoto T
Versatile strategy for isolating TALEN-mediated knockout mutants in Caenorhabditis elegans.
Dev Growth Differ, 56: 78-85, 2014
Sakane Y, Sakuma T, Kashiwagi K,Kashiwagi A, Yamamoto T and Suzuki K
Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of TALENs.
Dev Growth Differ, 56: 108-114, 2014
Hosoi S, Sakuma T, Sakamoto N and Yamamoto T
Targeted mutagenesis in sea urchin embryos using TALENs.
Dev Growth Differ, 56: 92-97, 2014
Hayashi T, Sakamoto K, Sakuma T, Yokotani N, Inoue T, Kawaguchi E, Agata K, Yamamoto T and Takeuchi T
TALENs efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration.
Dev Growth Differ, 56: 115-121, 2014
Kondo T, Sakuma T, Wada H, Akimoto A, Yamamoto T and Hayashi S
TALEN-induced gene knock out in Drosophila.
Dev Growth Differ, 56: 86-91, 2014
Nakagawa Y, Yamamoto T, Suzuki KI, Araki K, Takeda N, Ohmuraya M and Sakuma T
Screening methods to identify TALEN-mediated knockout mice.
Exp Anim, 63: 79-84, 2014

【総説・著書】

Yamamoto T and Nakamura H
Targeted genome editing.
Dev Growth Differ, 56: 1, 2014
Sakuma T and Woltjen K
Nuclease-mediated genome editing: At the front-line of functional genomics technology.
Dev Growth Differ, 56: 2-13, 2014
Hanada H, Kashiwagi K, Suzuki K, Tazawa I, Yamamoto T and Kashiwagi A
Suppression of anuran metamorphosis by synthetic chemical compounds
Frogs: Genetic Diversity, Neural Development and Environmental Influences (Ed by Lambert H), 73-88, 2014
山本 卓
ゲノム編集総説、ゲノム編集ハンドブック(コスモバイオ)、p2-3 (2014)
坂本尚昭山本 卓
部位特異的ヌクレアーゼを利用したゲノム編集技術、整形・災害外科、Vol.57、p1609-1614 (2014)
佐久間哲史山本 卓
高活性型Platinum TALENおよびCRISPR/Cas9を用いたゲノム編集、DNA鑑定、Vol.6 (2014)
山本 卓坂本尚昭佐久間哲史
部位特異的ヌクレアーゼを利用したゲノム編集、月刊バイオインダストリー, 2014年11月号 (2014)
佐久間哲史山本 卓
ゲノム編集による遺伝子改変技術、THE LUNG perspectives、Vol.22(4)、p71-75 (2014)
佐久間哲史
動物におけるゲノム編集技術の最前線、九州実験動物雑誌、Vol.30、p19-23 (2014)
山本 卓坂本尚昭佐久間哲史
部位特異的ヌクレアーゼを基盤とするゲノム編集技術、ウイルス、Vol.64(1)、75-82 (2014)
佐久間哲史
ゲノム編集技術ツールの開発の歴史、実験医学別冊「今すぐ始めるゲノム編集」、p8-13 (2014)
佐久間哲史山本 卓
ゲノム編集の原理と応用、実験医学別冊「今すぐ始めるゲノム編集」、p14-20 (2014)
佐久間哲史山本 卓
TALENやCRISPR/Cas9を自作するには、実験医学別冊「今すぐ始めるゲノム編集」、p23-28 (2014)
佐久間哲史山本 卓
TALENやCRISPR/Cas9の活性評価法と変異の検出法、実験医学別冊「今すぐ始めるゲノム編集」、p29-35 (2014)
佐久間哲史山本 卓
TALENやCRISPR/Cas9によるターゲティング戦略、実験医学別冊「今すぐ始めるゲノム編集」、p36-41 (2014)
佐久間哲史
ゲノム編集ツールの作製法、実験医学別冊「今すぐ始めるゲノム編集」、p46-61 (2014)
佐久間哲史
CRISPR/Cas9ダブルニッカーゼを用いた遺伝子改変法、実験医学別冊「今すぐ始めるゲノム編集」、p120-121 (2014)
林 利憲、坂根佑人、竹内 隆、鈴木賢一
ゲノム編集ツールの作製法、実験医学別冊「今すぐ始めるゲノム編集」、p180-188 (2014)
鈴木賢一佐久間哲史山本 卓</dt>
ゲノム編集技術を用いた個体レベルの機能解析:ツメガエルを例に、比較内分泌学、Vol.40、p35-36 (2014)
李 紅梅、佐久間哲史、堀田秋津、山本 卓
TALENによる遺伝子ターゲティング、実験医学別冊「ES・iPS細胞実験スタンダード」、p324-336 (2014)
山本 卓佐久間哲史鈴木賢一坂本尚昭
部位特異的ヌクレアーゼによるゲノム編集と動物における利用、生物の科学「遺伝」、Vol.68(2)、p130-134 (2014)
山本 卓佐久間哲史坂本尚昭
人工ヌクレアーゼを用いた培養細胞や動物でのゲノム編集、細胞、Vol.46(2)、p92-95 (2014)
坂本 尚昭, 粟津 暁紀
インスレーターとクロマチン構造, 生体の科学・特集「生命動態システム科学」,65, 412-413 (2014)

【特許】

鈴木賢一坂根祐人佐久間哲史山本 卓
核酸挿入用ベクター、PCT/JP2014/079515 (2014)

 

2013年

【原著論文】

Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S and Yamamoto T
Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity.
Scientific Reports, 3: 3379, 2013 日刊工業新聞、日経バイオテクonline
Kato T, Miyata K, Sonobe M, Yamashita S, Tamano M, Miura K, Kanai Y, Miyamoto S, Sakuma T, Yamamoto T, Inui M, Kikusui T, Asahara H, Takada S
Production of Sry knockout mouse using TALEN via oocyte injection.
Scientific Reports, 3: 3136, 2013 Nature Japan注目の論文、BioTechnique誌
Sakuma T, Hosoi S, Woltjen K, Suzuki KI, Kashiwagi K, Wada H, Ochiai H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A, Hayashi S and Yamamoto T
Efficient TALEN construction and evaluation methods for human cell and animal applications.
Genes Cells, 18: 315-326, 2013 Genes to Cellsの2013年のMost AccesedとMost citedのArticle
Mashimo T, Kaneko T, Sakuma T, Kobayashi J, Kunihiro Y, Voigt B, Yamamoto T and Serikawa T
Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes.
Scientific Reports, 3, 1253, 2013 中国新聞、京都新聞、日刊工業新聞、日経バイオテクonline、Nature Japan注目の論文
Mitsunaga-Nakatsubo K, Akimoto Y, Kusunoki S and Kawakami H.
Novel structure of hepatic extracellular matrices containing arylsulfatase A.
Okajimas Folia Anat Jpn, 90: 17-22, 2013
Suzuki KI, Isoyama Y, Kashiwagi K, Sakuma T, Ochiai H, Sakamoto N, Furuno N, Kashiwagi A and Yamamoto T
High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos.
Biology Open, 2: 448-452, 2013 BiO’s top five most-read articles for 2013
Hisano Y, Ota S, Arakawa S, Muraki M, Kono N, Oshita K, Sakuma T, Tomita M, Yamamoto T, Okada Y and Kawahara A
Quantitative assay for TALEN activity at endogenous genomic loci.
Biology Open, 2: 363-367, 2013 BiO’s top five most-read articles for 2013
Oulhen N, Yoshida T, Yajima M, Song J, Sakuma T, Sakamoto N, Yamamoto T and Wessel GM
The 3’UTR of Nanos2 directs enrichment in the germ cell lineage of the sea urchin.
Dev Biol, 377: 275-283, 2013
Ansai S, Sakuma T, Yamamoto T, Ariga H, Uemura N, Takahashi R and Kinoshita M.
Efficient targeted mutagenesis in medaka using custom-designed transcription activator-like effector nucleases (TALENs).
Genetics, 193: 739-749, 2013
Hozumi A, Yoshida R, Horie T, Sakuma T, Yamamoto T and Sasakura Y
Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome.
Dev Biol, 37: 79-91, 2013

【総説・著書】

山本 卓佐久間哲史坂本尚昭
ゲノム編集を利用した培養細胞や動物における標的遺伝子の改変、血管、Vol.36、p123-128 (2013)
山本 卓
高活性型TALENの開発と様々な動物におけるゲノム編集、関西実験動物研究会会報、Vol.35、p75-78 (2013)
佐久間哲史山本 卓
人工ヌクレアーゼを用いたゲノム編集研究の歴史と現状、DNA鑑定、Vol.5、p1-8 (2013)
佐久間哲史山本 卓
ゲノム編集技術を用いた遺伝子改変および染色体改変動物について、LABIO21、Vol.54、p16-18 (2013)
坂本尚昭山本 卓
棘皮動物のウニ、生物工学会誌、Vol.91(8)、p473-476 (2013)
山本 卓佐久間哲史鈴木賢一坂本尚昭
動物におけるゲノム編集技術の現状と可能性、バイオサイエンスとインダストリー、Vol.71(4)、p365-368 (2013)
山本 卓
ゲノム編集革命「基礎の基礎」、細胞工学、Vol.32(5)、p506-509 (2013)
佐久間哲史鈴木賢一坂本尚昭山本 卓
TALENの効率的な作製と動物個体への応用、細胞工学、Vol.32(5)、p510-514 (2013)
落合 博、柴田達夫、山本 卓
ゲノム編集技術を用いた遺伝子発現の定量的イメージング、細胞工学、Vol.32(5)、p538-542 (2013)
佐久間哲史
RNA誘導型ヌクレアーゼ:CRISPR/Casシステムによるゲノム編集、細胞工学、Vol.32(5)、p515-517 (2013)
山本 卓佐久間哲史坂本尚昭
標的遺伝子の改変を可能にする人工ヌクレアーゼの開発、化学工業、Vol.64(4)、p260-264 (2013)
落合 博、佐久間哲史、松浦伸也、山本 卓
TALE nuclease (TALEN)を用いた培養細胞におけるゲノム編集、実験医学、Vol.31(1)、p95-100 (2013)

【特許】

鈴木賢一坂根祐人佐久間哲史山本 卓
核酸挿入用ベクター、特願2013-230349 (2013)
佐久間哲史、落合 博、松浦伸也、宮本達雄、山本卓
DNA結合ドメインを含むポリペプチド、特願2013-166768 (2013)
佐久間哲史徳増大輔坂本尚昭山本 卓
ヌクレアーゼを発現させるためのベクターおよびベクターセット、ならびに変異導入を受けた細胞の取得方法、特願2013-098724 (2013)
中村崇裕、八木祐介、大川恭行、佐久間哲史山本 卓
PPRモチーフを利用したDNA結合性蛋白質の設計方法及びその利用、特願2013-88940 (2013)

 

2012年

Watanabe T, Ochiai H, Sakuma T, Horch H, Hamaguchi N, Nakamura T, Bando T, Ohuchi H, Yamamoto T, Noji S and Mito T
Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases.
Nat Commun, 3: 1017, 2012 朝日新聞、読売新聞、中国新聞、日経バイオテクonline、Nature Japan注目の論文
Ochiai H, Sakamoto N, Fujita K, Nishikawa M, Suzuki KI, Matsuura S, Miyamoto T, Sakuma T, Shibata T and Yamamoto T
Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos.
Proc Natl Acad Sci U S A, 109: 10915-10920, 2012 中国新聞、日経バイオテクonline
Song X, Sato Y, Felemban A, Ito A, Hossain M, Ochiai H, Yamamoto T, Sekiguchi K, Tanaka H and Ohta K
Equarin is involved as an FGF signaling modulator in chick lens differentiation.
Dev Biol, 368: 109-117, 2012
Ansai S, Ochiai H, Kanie Y, Kamei Y, Goh Y, Kitano T, Yamamoto T and Kinoshita M
Targeted disruption of exogenous EGFP gene in medaka using zinc-finger nucleases.
Dev Growth Differ, 54: 546-556, 2012
Kawai N, Ochiai H, Sakuma T, Yamada L, Sawada H, Yamamoto T and Sasakura Y
Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases.
Dev Growth Differ, 54: 535-545, 2012 Faculty of 1000 Recommended
Takagi H, Inai Y, Watanabe S, Tatemoto S, Yajima M, Akasaka K, Yamamoto T and Sakamoto N
Nucleosome exclusion from the interspecies conserved central AT-rich region of the Ars insulator.
J Biochem, 151: 75-87, 2012 JB論文賞
佐久間哲史、坂本尚昭、山本 卓
人工ヌクレアーゼを用いたゲノム編集、Medical Science Digest、Vol38(8), p328-329 (2012)

 

2011年

Sakuma T, Ohnishi K, Fujita K, Ochiai H, Sakamoto N and Yamamoto T
HpSumf1 is involved in the activation of sulfatases responsible for regulation of skeletogenesis during sea urchin development.
Dev Genes Evol221: 157-166, 2011
山本 卓、坂本尚昭、佐久間哲史、野地澄晴
新規DNA結合ドメインおよびそれを含む新規DNA切断酵素、特願2011-242250 (2011)

 

2010年

Ochiai H, Fujita K, Suzuki K, Nishikawa M, Shibata T, Sakamoto N and Yamamoto T
Targeted mutagenesis in the sea urchin embryo using zinc-finger nucleases.
Genes Cells15: 875-885, 2010
Okamitsu Y, Yamamoto T, Fujii T, Ochiai H and Sakamoto N
Dicer is required for the normal development of sea urchin, Hemicentrotus pulcherrimus.
Zoolog Sci27: 477-486, 2010
Yajima M, Umeda R, Fuchikami T, Kataoka M, Sakamoto N, Yamamoto T and Akasaka K
Implication of HpEts in gene regulatory networks responsible for specification of sea urchin skeletogenic primary mesenchyme cells
Zoolog Sci27: 638-646, 2010
Fujita K, Takechi E, Sakamoto N, Sumiyoshi N, Izumi S, Miyamoto T, Matsuura S, Tsurugaya T, Akasaka K and Yamamoto T
HpSulf, a heparan sulfate 6-O-endosulfatase, is involved in the regulation of VEGF signaling during sea urchin development.
Mech Dev127: 235-245, 2010
Fujita K, Teramura N, Hattori S, Irie S, Mitsunaga-Nakatsubo K, Akimoto T, Sakamoto N, Yamamoto T and Akasaka K
Mammalian arlysulfatase A functions as a novel component of the extracellular matrix.
Connect Tissue Res51: 388-396, 2010

 

2009年

Karasawa K, Sakamoto N, Fujita K, Ochiai H, Fujii T, Akasaka K and Yamamoto T
Suppressor of Hairless (Su(H)) is required for foregut development in the sea urchin embryo.
Zoolog Sci26: 686-690, 2009
Fujii T, Sakamoto N, Ochiai H, Fujita K, Okamitsu Y, Sumiyoshi N, Minokawa T and Yamamoto T
Role of the nanos homolog during sea urchin development.
Dev Dyn238: 2511-2521, 2009
Mitsunaga-Nakatsubo K, Kusunoki S, Kawakami H, Akasaka K and Akimoto Y
Cell-surface arylsulfatase A and B on sinusoidal endothelial cells, hepatocytes, and Kupffer cells in mammalian livers.
Med Mol Morphol42: 63-69, 2009
Mitsunaga-Nakatsubo K, Akimoto Y, Kawakami H and Akasaka K
Sea urchin arylsulfatase, an extracellular matrix component, is involved in gastrulation during embryogenesis.
Dev Genes Evol219: 281-288, 2009

 

2008年

Ochiai H, Sakamoto N, Suzuki K, Akasaka K and Yamamoto T
The Ars insulator facilitates I-SceI meganuclease-mediated transgenesis in the sea urchin embryo.
Dev Dyn237: 2475-2482, 2008
Hanai K, Furuhashi H, Yamamoto T, Akasaka K and Hirose S
RSF Governs Silent Chromatin Formation via Histone H2Av Replacement.
PLoS genet4: e1000011, 2008
Ochiai H, Sakamoto N, Momiyama A, Akasaka K and Yamamoto T
Analysis of cis-regulatory elements controlling spatio-temporal expression of T-brain gene in sea urchin, Hemicentrotus pulcherrimus.
Mech Dev125: 2-17, 2008

 

2007年

Yamamoto T, Kawamoto R, Fujii T, Sakamoto N and Shibata T
DNA variations within the sea urchin Otx gene enhancer.
FEBS Lett581: 5234-5240, 2007

 

2006年

Sakamoto N, Yamamoto T and Akasaka K
Involvement of nuclear matrix and chromatin loop formation in the function of insulators.
Chapter14 of review book, DNA Structure, Chromatin and Gene Expression, 193-205, 2006
Watanabe S, Watanabe S, Sakamoto N, Sato M and Akasaka K
Functional analysis of the sea urchin-derived arylsulfatase (Ars)-element in mammalian cells.
Genes Cells11: 1009-1021, 2006
Fujii T, Mitsunaga-Nakatsubo K, Saito I, Iida H, Sakamoto N, Akasaka K and Yamamoto T
Developmental expression of HpNanos, the Hemicentrotus pulcherrimus homologue of nanos.
Gene Expr Patterns6: 572-577, 2006
Tagashira H, Shimotori T, Sakamoto N, Katahira M, Miyanoiri Y, Yamamoto T, Mitsunaga-Nakatsubo K, Shimada H, Kusunoki S and Akasaka K
Unichrom, a novel nuclear matrix protein, binds to the Ars insulator and canonical MARs.
Zoolog Sci23: 9-21, 2006

 

2005年

Ohtsuki, T., Otsuki, M., Murakami, Y., Maekawa, T., Yamamoto, T., Akasaka, K., Takeuchi, S. and Takahashi S
Organ-specific and age-dependent expression of insulin-like growth factor-I (IGF-I) mRNA variants: IGF-IA and IB mRNAs in the mouse.
Zoolog Sci22: 1011-1021, 2005
Yamazaki A, Kawabata R, Shiomi K, Amemiya S, Sawaguchi M, Mitsunaga-Nakatsubo K and Yamaguchi M
The micro1 gene is necessary and sufficient for micromere differentiation and mid/hindgut-inducing activity in the sea urchin embryo.
Dev Genes Evol215: 450-459, 2005

 

2004年

Moritani K, Tagashira H, Shimotori T, Sakamoto N, Tanaka S, Takata K, Mitsunaga-Nakatsubo K, Bojiiwa Y, Yamamoto T, Shimada H and Akasaka K
A new G-stretch-DNA-binding protein, Unichrom, displays cell-cycle-dependent expression in sea urchin embryos.
Dev Growth Differ46: 335-341, 2004
Hayashibara Y, Mitsunaga-Nakatsubo K, Sakamoto N, Shimotori T, Akasaka K and Yamamoto T
The Otx binding site is required for the activation of HpOtxL mRNA expression in the sea urchin, Hemicentrotus pulcherrimus.
Dev Growth Differ46: 61-67, 2004

 

2003年

Kurita M, Kondoh H, Mitsunaga-Nakatsubo K, Shimotori T, Sakamoto N, Yamamoto T, Shimada H, Takata K and Akasaka K
Utilization of a particle gun DNA introduction system for the analysis of cis-regulatory elements controlling the spatial expression pattern of the arylsulfatase gene ( HpArs) in sea urchin embryos.
Dev Genes Evol213: 44-49, 2003

 

2002年

Fuchikami T, Mitsunaga-Nakatsubo K, Amemiya S, Hosomi T, Watanabe T, Kurokawa D, Kataoka M, Harada Y, Satoh N, Kusunoki S, Takata K, Shimotori T, Yamamoto T, Sakamoto N, Shimada H and Akasaka K
T-brain homologue (HpTb) is involved in the archenteron induction signals of micromere descendant cells in the sea urchin embryo.
Development129: 5205-5216, 2002

 

2000年

Kiyama T, Sasai K, Takata K, Mitsunaga-Nakatsubo K, Shimada H and Akasaka K
CAAT sites are required for the activation of the H. pulcherrimus Ars gene by Otx.
Dev Genes Evol210: 583-590, 2000
Ogawa M, Akasaka K, Mitsunaga-Nakatsubo K and Shimada H
Sox regulates transcription of the sea urchin arylsulfatase gene.
Dev Growth Differ42: 429-435, 2000
Mitsunaga-Nakatsubo K, Kawasaki T, Takeda K, Akasaka K and Shimada H
Lim1-related homeobox gene (HpLim1) expressed in sea urchin embryo.
Zygote8 Suppl 1: S71-72, 2000
Kurokawa D, Kitajima T, Mitsunaga-Nakatsubo K, Amemiya S, Shimada H and Akasaka K
HpEts implicated in primary mesenchyme cell differentiation of the sea urchin (Hemicentrotus pulcherrimus) embryo.
Zygote8 Suppl 1: S33-34, 2000

 

1999年

Kurokawa D, Kitajima T, Mitsunaga-Nakatsubo K, Amemiya S, Shimada H and Akasaka K
HpEts, an ets-related transcription factor implicated in primary mesenchyme cell differentiation in the sea urchin embryo.
Mech Dev80: 41-52, 1999
Kawasaki T, Mitsunaga-Nakatsubo K, Takeda K, Akasaka K and Shimada H
Lim1 related homeobox gene (HpLim1) expressed in sea urchin embryos.
Dev Growth Differ41: 273-282, 1999
Ishii M, Mitsunaga-Nakatsubo K, Kitajima T, Kusunoki S, Shimada H and Akasaka K
Hbox1 and Hbox7 are involved in pattern formation in sea urchin embryos.
Dev Growth Differ41: 241-252, 1999
Akasaka K, Nishimura A, Takata K, Mitsunaga K, Mibuka F, Ueda H, Hirose S, Tsutsui K and Shimada H
Upstream element of the sea urchin arylsulfatase gene serves as an insulator.
Cell Mol Biol45: 555-565, 1999

 

1998年

Koike H, Akasaka K, Mitsunaga-Nakatsubo K and Shimada H
Proximal cis-regulatory elements of sea urchin arylsulfatase gene.
Dev Growth Differ40: 537-544, 1998
Mitsunaga-Nakatsubo K, Akasaka K, Sakamoto N, Takata K, Matsumura Y, Kitajima T, Kusunoki S and Shimada H
Differential expression of sea urchin Otx isoform (hpOtxE and HpOtxL) mRNAs during early development.
Int J Dev Biol42: 645-651, 1998
Kiyama T, Akasaka K, Takata K, Mitsunaga-Nakatsubo K, Sakamoto N, Shimada H
Structure and function of a sea urchin orthodenticle-related gene (HpOtx).
Dev Biol193: 139-145, 1998
Mitsunaga-Nakatsubo K, Akasaka K, Akimoto Y, Akiba E, Kitajima T, Tomita M, Hirano H and Shimada H
Arylsulfatase exists as non-enzymatic cell surface protein in sea urchin embryos.
J Exp Zool280: 220-230, 1998

 

1997年

Kurokawa D, Akasaka K, Mitsunaga-Nakatsubo K and Shimada H
Cloning of cyclin E cDNA of the sea urchin, Hemicentrotus pulcherrimus.
Zoolog Sci14: 791-794, 1997
Morokuma J, Akasaka K, Mitsunaga-Nakatsubo K and Shimada H
A cis-regulatory element within the 5′ flanking region of arylsulfatase gene of sea urchin, Hemicentrotus pulcherrimus.
Dev Growth Differ39: 469-476, 1997
Akasaka K, Uemoto H, Wilt F, Mitsunaga-Nakatsubo K and Shimada H
Oral-aboral ectoderm differentiation of sea urchin embryos is disrupted in response to calcium ionophore.
Dev Growth Differ39: 373-379, 1997
Sakamoto N, Akasaka K, Mitsunaga-Nakatsubo K, Takata K, Nishitani T and Shimada H
Two isoforms of orthodenticle-related proteins (HpOtx) bind to the enhancer element of sea urchin arylsulfatase gene.
Dev Biol181: 284-295, 1997

 

1996年

Mitsunaga-Nakatsubo K, Yamazaki K, Hatoh-Okazaki M, Kawashita H, Okamura C, Akasaka K, Shimada H and Yasumasu I
cDNA cloning of Na+, K(+)-ATPase alpha-subunit from embryos of the sea urchin, Hemicentrotus pulcherrimus.
Zoolog Sci13: 833-841, 1996
Sakamoto N, Akasaka K, Yamamoto T and Shimada H
A triplex DNA structure of the polypyrimidine: polypurine stretch in the 5′ flanking region of the sea urchin arylsulfatase gene.
Zoolog Sci13: 105-109, 1996

 

1994年

Yamamoto T, Akasaka K, Irie S and Shimada H
A long polypyrimidine:polypurine sequence in 5′ flanking region of arylsulfatase gene of sea urchin embryo.
Int J Dev Biol38: 337-344, 1994