{"id":374,"date":"2021-10-30T13:11:27","date_gmt":"2021-10-30T13:11:27","guid":{"rendered":"https:\/\/smg.hiroshima-u.ac.jp\/?page_id=374"},"modified":"2021-10-31T08:08:07","modified_gmt":"2021-10-31T08:08:07","slug":"genome-editing","status":"publish","type":"page","link":"https:\/\/smg.hiroshima-u.ac.jp\/index.php\/genome-editing\/","title":{"rendered":"\u90e8\u4f4d\u7279\u7570\u7684\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u306b\u3088\u308b\u30b2\u30ce\u30e0\u7de8\u96c6"},"content":{"rendered":"<div id=\"main\">\n<div id=\"research\">\n<p>\u8fd1\u5e74\u3001\u69d8\u3005\u306a\u751f\u7269\u7a2e\u306b\u304a\u3044\u3066\u6a19\u7684\u907a\u4f1d\u5b50\u3092\u6539\u5909\u3059\u308b\u6280\u8853\u3068\u3057\u3066\u3001\u4eba\u5de5\u30cc\u30af\u30ec\u30a2\u30fc\u30bc(ZFN\u3084TALEN)\u3092\u5229\u7528\u3057\u305f\u201c\u30b2\u30ce\u30e0\u7de8\u96c6\u201d\u304c\u6ce8\u76ee\u3055\u308c\u3066\u3044\u308b\u3002\u4eba\u5de5\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u306f\u3001DNA\u306b\u7279\u7570\u7684\u306b\u7d50\u5408\u3059\u308b\u30c9\u30e1\u30a4\u30f3\u3068\u3001\u5236\u9650\u9175\u7d20FokI\u306eDNA\u5207\u65ad\u30c9\u30e1\u30a4\u30f3\u3092\u9023\u7d50\u3055\u305b\u305f\u30ad\u30e1\u30e9\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308b\u3002\uff12\u3064\u306e\u4eba\u5de5\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u304c\u8fd1\u63a5\u3059\u308b\u6a19\u7684\u914d\u5217\u306b\u7d50\u5408\u3059\u308b\u3068DNA\u5207\u65ad\u30c9\u30e1\u30a4\u30f3\u304c\uff12\u91cf\u4f53\u3068\u306a\u308aDNA\u3092\u5207\u65ad\u3059\u308b\u3002\u5207\u65ad\u3055\u308c\u305fDNA\u306f\u3001\u76f8\u540c\u7d44\u63db\u3048\u3042\u308b\u3044\u306f\u975e\u76f8\u540c\u672b\u7aef\u9023\u7d50\u306b\u3088\u308a\u4fee\u5fa9\u3055\u308c\u308b\u304c\u3001\u3053\u306e\u6642\u306b\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u3092\u6539\u5909\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3068\u306a\u308b\u3002\u6a19\u7684\u914d\u5217\u306e\u9078\u629e\u304c\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068\u304b\u3089\u6b21\u4e16\u4ee3\u306e\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u6280\u8853\u3068\u3057\u3066\u6ce8\u76ee\u3055\u308c\u3001\u69d8\u3005\u306a\u52d5\u7269\u3001\u690d\u7269\u3001\u54fa\u4e73\u985e\u57f9\u990a\u7d30\u80de\uff08ES\u7d30\u80de\u3084iPS\u7d30\u80de\u3092\u542b\u3080\uff09\u306b\u304a\u3044\u3066\u6210\u529f\u4f8b\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u308b\u3002\u4eba\u5de5\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u306b\u52a0\u3048\u3066\u30012013\u5e74\u59cb\u3081\u306b\u7d30\u83cc\u3084\u53e4\u7d30\u83cc\u306e\u7372\u5f97\u514d\u75ab\u7cfb\u3092\u5229\u7528\u3057\u305fCRISPR\uff0fCas9\u30b7\u30b9\u30c6\u30e0\u304c\u5831\u544a\u3055\u308c\u3001\u4eca\u5f8c\u306fCRISPR\/Cas\u304c\u4e2d\u5fc3\u7684\u306a\u30c4\u30fc\u30eb\u3068\u3057\u3066\u5229\u7528\u3055\u308c\u308b\u3068\u4e88\u60f3\u3055\u308c\u308b\u3002<\/p>\n<p>\u6211\u3005\u306f\u3001\u30aa\u30fc\u30d7\u30f3\u30ea\u30bd\u30fc\u30b9\u3092\u5229\u7528\u3057\u305f\u4eba\u5de5\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u306e\u4f5c\u88fd\u65b9\u6cd5\u3092\u78ba\u7acb\u3057\u3001\u30a6\u30cb\u80da\u3092\u30e2\u30c7\u30eb\u3068\u3057\u3066Zinc finger nucleases (ZFNs)\u306b\u3088\u308b\u907a\u4f1d\u5b50\u7834\u58ca\uff08Ochiai et al., 2010\uff09\u304a\u3088\u3073\u30ec\u30dd\u30fc\u30bf\u30fc\u907a\u4f1d\u5b50\u306e\u30ce\u30c3\u30af\u30a4\u30f3\u3092\u884c\u3063\u3066\u304d\u305f\uff08Ochiai et al., 2012\uff09\u3002\u52a0\u3048\u3066\u3001\u690d\u7269\u7d30\u83ccXanthomonas\u7531\u6765\u306eTranscription activator like effector(TALE)\u306eDNA\u7d50\u5408\u30c9\u30e1\u30a4\u30f3\u3092\u5229\u7528\u3057\u305fTALE nucleases (TALENs)\u306b\u3088\u308b\u907a\u4f1d\u6539\u5909\u6280\u8853\u3092\u78ba\u7acb\u3057(Sakuma et al., 2013)\u3001\u69d8\u3005\u306a\u751f\u7269(\u30e9\u30c3\u30c8\u3001\u30de\u30a6\u30b9\u3001\u30de\u30fc\u30e2\u30bb\u30c3\u30c8\u3001\u30db\u30e4\u3001\u30a6\u30cb\u3001\u30b7\u30e7\u30a6\u30b8\u30e7\u30a6\u30d0\u30a8\u3001\u30b3\u30aa\u30ed\u30ae\u3001\u30a6\u30cb\u3001\u690d\u7269\uff09\u3067\u306e\u6a19\u7684\u907a\u4f1d\u5b50\u306e\u6539\u5909\u3092\u9032\u3081\u3066\u304d\u305f\u3002\u3055\u3089\u306b\u3001CRISPR\/Cas9\u306e\u30de\u30eb\u30c1\u30ac\u30a4\u30c9\u30b7\u30b9\u30c6\u30e0\u3092\u78ba\u7acb\u3057\u3001\u57f9\u990a\u7d30\u80de\u306b\u304a\u3051\u308b\u8907\u6570\u540c\u6642\u6539\u5909\u304c\u53ef\u80fd\u306a\u3053\u3068\u3092\u8a3c\u660e\u3057\u305f(Sakuma et al., 2014)\u3002<\/p>\n<p class=\"noindent\">Ochiai et al., Targeted mutagenesis in the sea urchin embryo using zinc-finger nucleases, <em>Genes to Cells<\/em>, <strong>15<\/strong>:875-885 (2010)<\/p>\n<p class=\"noindent\">Ochiai et al., Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos, <em>Proc Natl Acad Sci U S A<\/em>, <strong>109<\/strong>: 10915-10920 (2012)<\/p>\n<p class=\"noindent\">Sakuma et al., Efficient TALEN construction and evaluation methods for human cell and animal applications, <em>Genes to Cells<\/em>, <strong>18<\/strong>: 315-326 (2013)<\/p>\n<p class=\"noindent\">Sakuma et al., Multiplex genome engineering in human cells using all-in-one CRISPR\/Cas9 vector system, <em>Scientific Reports<\/em>, <strong>4<\/strong>: 5400 (2014)<\/p>\n<p style=\"text-align: center;\"><a href=\"https:\/\/smg.hiroshima-u.ac.jp\/wp-content\/uploads\/2021\/10\/custamized_nuc.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-371\" src=\"https:\/\/smg.hiroshima-u.ac.jp\/wp-content\/uploads\/2021\/10\/custamized_nuc.jpg\" alt=\"\" width=\"496\" height=\"194\" srcset=\"https:\/\/smg.hiroshima-u.ac.jp\/wp-content\/uploads\/2021\/10\/custamized_nuc.jpg 640w, https:\/\/smg.hiroshima-u.ac.jp\/wp-content\/uploads\/2021\/10\/custamized_nuc-300x117.jpg 300w\" sizes=\"auto, (max-width: 496px) 100vw, 496px\" \/><\/a><span style=\"color: #0000ff; font-size: small;\">\u56f3\uff11\uff1a\u4eba\u5de5\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u306b\u3088\u308bDNA\u4e8c\u672c\u9396\u5207\u65ad<\/span><\/p>\n<p style=\"text-align: center;\"><a href=\"https:\/\/smg.hiroshima-u.ac.jp\/wp-content\/uploads\/2021\/10\/mutagenesis.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-372\" src=\"https:\/\/smg.hiroshima-u.ac.jp\/wp-content\/uploads\/2021\/10\/mutagenesis.jpg\" alt=\"\" width=\"494\" height=\"350\" srcset=\"https:\/\/smg.hiroshima-u.ac.jp\/wp-content\/uploads\/2021\/10\/mutagenesis.jpg 640w, https:\/\/smg.hiroshima-u.ac.jp\/wp-content\/uploads\/2021\/10\/mutagenesis-300x212.jpg 300w\" sizes=\"auto, (max-width: 494px) 100vw, 494px\" \/><\/a><span style=\"color: #0000ff; font-size: small;\">\u56f3\uff12\uff1a\u5207\u65ad\u90e8\u4f4d\u306b\u304a\u3051\u308b\u5909\u7570\u5c0e\u5165<\/span><\/p>\n<p style=\"text-align: center;\"><a href=\"https:\/\/smg.hiroshima-u.ac.jp\/wp-content\/uploads\/2021\/10\/mutations.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-373\" src=\"https:\/\/smg.hiroshima-u.ac.jp\/wp-content\/uploads\/2021\/10\/mutations.jpg\" alt=\"\" width=\"446\" height=\"371\" srcset=\"https:\/\/smg.hiroshima-u.ac.jp\/wp-content\/uploads\/2021\/10\/mutations.jpg 577w, https:\/\/smg.hiroshima-u.ac.jp\/wp-content\/uploads\/2021\/10\/mutations-300x250.jpg 300w\" sizes=\"auto, (max-width: 446px) 100vw, 446px\" \/><\/a><span style=\"color: #0000ff; font-size: small;\">\u56f3\uff13\uff1a\u4eba\u5de5\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u306b\u3088\u308a\u5c0e\u5165\u3055\u308c\u305f\u5909\u7570<\/span><\/p>\n<p class=\"noindent\" align=\"right\"><a href=\"https:\/\/smg.hiroshima-u.ac.jp\/index.php\/research\/\"><img decoding=\"async\" style=\"vertical-align: middle;\" src=\"https:\/\/smg.hiroshima-u.ac.jp\/wp-content\/uploads\/2021\/10\/index.jpg\" border=\"0\" \/>\u7814\u7a76\u5185\u5bb9\u3078\u623b\u308b<\/a><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd1\u5e74\u3001\u69d8\u3005\u306a\u751f\u7269\u7a2e\u306b\u304a\u3044\u3066\u6a19\u7684\u907a\u4f1d\u5b50\u3092\u6539\u5909\u3059\u308b\u6280\u8853\u3068\u3057\u3066\u3001\u4eba\u5de5\u30cc\u30af\u30ec&#46;&#46;&#46;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-374","page","type-page","status-publish","hentry"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/smg.hiroshima-u.ac.jp\/index.php\/wp-json\/wp\/v2\/pages\/374","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/smg.hiroshima-u.ac.jp\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/smg.hiroshima-u.ac.jp\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/smg.hiroshima-u.ac.jp\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/smg.hiroshima-u.ac.jp\/index.php\/wp-json\/wp\/v2\/comments?post=374"}],"version-history":[{"count":11,"href":"https:\/\/smg.hiroshima-u.ac.jp\/index.php\/wp-json\/wp\/v2\/pages\/374\/revisions"}],"predecessor-version":[{"id":407,"href":"https:\/\/smg.hiroshima-u.ac.jp\/index.php\/wp-json\/wp\/v2\/pages\/374\/revisions\/407"}],"wp:attachment":[{"href":"https:\/\/smg.hiroshima-u.ac.jp\/index.php\/wp-json\/wp\/v2\/media?parent=374"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}